||The GPEx® gene insertion technology
produces mammalian cell lines - typically CHO or 293 (HEK) cells
- that deliver higher yields of target proteins than any conventional
system based on transfection or electroporation.
One reason for these higher yields is that the GPEx® retrovector
targets high-expressing chromosomal matrix attachment sites
in the target cell genome, leading to markedly higher expression
of the gene in transduced cells compared with other systems.
Another important reason is that the optimization of GPEx® cell
lines employs an iterative insertion process that drives
up the inserted gene copy number and proportionally increases
protein expression levels. The optimized cell line may therefore
have a dozen or more copies of the desired gene, all stably
inserted and expressing the target protein.